Information

Protocols, submission forms and further information.

Reverse Phase Protein Arrays:

Sample Submission Form
HTPU RPPA Submission Form (277.22 KB / DOCX)
Further information on using our service and sample preparation
Graphs
Example of 12 sample and 36 sample array outputs
Simplified schematic of workflow
Simplified schematic of workflow

Forward Phase Antibody Arrays:

Sample Submission Form
Further information on using our service and sample preparation
Example of the layout of a 64 antibody array
Example of the layout of a 64 antibody array

nCounter:

Sample Submission Form
Further information on using our service and sample guidelines

GeoMx DSP:

Sample Submission Form
GeoMx Submission Form (331 KB / DOCX)
Further information on using our service and sample preparation guidelines

 

  • Morphology marker staining should be optimised prior to submission to HTPU.
  • For protein workflows all antibodies should be conjugated.
  • For RNA workflows antibodies can be conjugated, non-conjugated or a mixture of both, however for non-conjugated the species must not be the same.
  • Nuclear stains can be provided by the facility: SYTO 13 (AF488) or SYTO 83 (AF532).
ChannelExcitation (peak/bandwidth)Emission (peak/bandwidth)
AF488 / FITC480 / 28 nm516 / 23 nm
AF532 / Cy3538 / 19 nm564 / 5 nm
AF594 / Texas Red588 / 19 nm623 / 30 nm
AF647 / Cy5645 / 19 nm683 / 30 nm

Further information on panels and technology GeoMx Digital Spatial Profiler | Bruker Spatial Biology

PamDx:

Sample submission Form
PamDx Submission Form (344.57 KB / DOCX)
Further information on using our service and sample preparation guidelines

 

For further information contact Jasper Snoep jsnoep@pamdx.com

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