Genome editing and targeted protein degradation Image Dr Andrew Wood - Reader and IGC Chancellor's Fellow Research in a Nutshell We develop technologies to manipulate the function of genes and proteins in physiological models of human disease, focusing on CRISPR and targeted protein degradation. Our laboratory applies these technologies to study the mechanisms through which the genome is propagated through cell division. Through collaboration, we also apply them more broadly to study other biological processes relevant to human disease. Our research is divided into three main themes: Degron Tagging Genome Editing Mitotic Chromosome Condensation Research Programme Image People Dr Andrew Wood Group Leader Abram Giller PhD Student Evelina Gudauskaite MRes student and Research Technician Emma Ramsey MRes Student Brianda Hernandez Moran Postdoctoral Scientist Dr Gillian Taylor Postdoctoral Scientist Molly Donald Research Technician Contact Andrew.J.Wood@ed.ac.uk Publications Krishna A, Meynert A, Kelder M, Ewing A, Sheraz S, Ferrer-Vaquer A, Grimes G, Becher H, Silk R, Semple C, Kendall T, Hadjantonakis A, Bird T, Marsh JA, Hohenstein P*, Wood AJ*, Ozdemir D* (2023) Mutational scanning reveals oncogenic CTNNB1 mutations have diverse effects on signalling and clinical traits. bioRxiv, https://doi.org/10.1101/2023.11.09.566307 Macdonald L, Taylor G, Brisbane J, Christodoulou E, Scott L, Von Kriegsheim A, Rossant J, Gu B*, Wood AJ* (2022) Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons. eLife Jun 23, 2022. https://doi.org/10.7554/eLife.77987 Kallimasioti-Pazi I, Chathoth K, Taylor G, Meynert A, Ballinger T, Kelder M, Sanli I, Lalevee S, Feil R, Wood AJ. (2018) Heterochromatin impedes CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair. PLoS Biology 16:e2005595 Therizols PT, Illingworth R, Courilleau C, Boyle S, Wood AJ, Bickmore WA. (2014) Chromatin decondensation is sufficient to alter nuclear positioning in embryonic stem cells. Science. 346:1238-1242. *Wood AJ, *Lo TW, *Zeitler B, Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Meyer BJ. (2011) Targeted genome editing across species using ZFNs and TALENs. Science. 333:30 Full publication list can be found on Research Explorer: Andrew Wood — University of Edinburgh Research Explorer Collaborations Dr Joe Marsh Professor Wendy Bickmore Professor Andrew Jackson Professor Javier Caceres Partners and Funders The Wellcome Trust The Royal Society Medical Research Council Scientific Themes genome stability, mutagenesis, chromosome structure, cell proliferation Technology Expertise Genome editing, flow cytometry, in vivo modelling, targeted protein degradation This article was published on 2024-09-23
