PamDx Kinase Profiling

Functional kinase assay measuring peptide phosphorylation by protein kinases.

HTPU Microarray Services are currently hosting a PamStation from PamDx, a unique technology making real-time measurement and understanding of kinase activity possible. PamDx’s functional phosphoproteomic assays bring a new dimension and additional information about how cell signalling works in various disease areas.

  • Phosphotyrosine kinase (PTK) arrays: 196 peptides
  • Serine-threonine kinase (STK) arrays: 144 peptides
  • PamChip® contains 4 identical peptide arrays
  • Peptides harbour phosphorylation sites derived from literature or computational predictions and are correlated with one or multiple upstream kinases
  • 12 samples per machine run
  • Typical input required: 1-5 µg (1 µg/ul in 10 µl aliquots)
  • Extraction buffer: M-PERTM Mammalian Extraction buffer with HALT Phosphatase Inhibitor cocktail & HALT Protease Inhibitor cocktail
  • Further information

For further information contact Jasper Snoep jsnoep@pamdx.com

PamDx Kinase Profiling Graph

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