Access to Fucci reagents

How to access Fucci biosensor reagents developed at the MRC Human Genetics Unit, University of Edinburgh.

Researchers at MRC HGU under the leadership of Dr Richard Mort, conducted research into Fucci biosensors resulting in the development of the Fucci2a bicistronic and Arl13b-Fucci2a tricistronic sensors.

These biosensors have been a valuable research reagent and can be accessed as described below

Where to access Fucci biosensor reagents

Cell Lines

Material

Paper to be cited if used

Reference

Actions

Flp-NIH3T3 Fucci2a Mort et al Cell Cycle 2014 Via UoE MTA - contact Pleasantine Mill PM to work with Richard Mort to ensure correct deposit of these cells and confirm with team
E14 MESC R26Fucci2aR Mort et al Cell Cycle 2014 Via UoE MTA - contact Pleasantine Mill PM to work with Richard Mort to ensure correct deposit of these cells
E14 MESC R26Fucci2a Mort et al Cell Cycle 2014 Via UoE MTA - contact Pleasantine Mill PM to work with Richard Mort to ensure correct deposit of these cells
Flp-NIH3T3 Arl13b-Fucci2a Ford et al Dev Cell 2018 Via UoE MTA - contact Pleasantine Mill PM to work with Richard Mort to ensure correct deposit of these cells
E14 R26Arl13b-Fucci2aR Ford et al Dev Cell 2018

Via UoE MTA - contact Pleasantine Mill

PM to work with Richard Mort to ensure correct deposit of these cells
E14 R26Arl13b-Fucci2a Ford et al Dev Cell 2018 Via UoE MTA - contact Pleasantine Mill PM to work with Richard Mort to ensure correct deposit of these cells

Plasmids

Material

Paper to be cited if used

Location

Reference

pCAG-Fucci2a Mort et al Cell Cycle 2014 Access via RIKEN dnaconda.riken.jp/search/RDB_clone/RDB13080
pCDNA5-Fucci2a Mort et al Cell Cycle 2014 Access via RIKEN dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16056
pRosa26-CAG-floxNeo-Fucci2a Mort et al Cell Cycle 2014 Access via RIKEN dnaconda.riken.jp/search/RDB_clone/RDB13/RDB13081
pCAG-Arl13bCerulean-Fucci2a Ford et al Dev Cell 2018 Access via RIKEN dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16059
pCDNA5-CAG-Arl13bCerulean-Fucci2a Ford et al Dev Cell 2018 Access via RIKEN dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16057
pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a Ford et al Dev Cell 2018 Access via RIKEN dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16058

Mouse Lines

Material

Paper to be cited if used

Location

Reference 1

Reference 2

R26Fucci2aR Mort et al Cell Cycle 2014 Access via RIKEN infrafrontier.eu/emma/strain-search/straindetails/?q=8395 knowledge.brc.riken.jp/resource/animal/card?brc_no=RBRC06511
R26Fucci2a Mort et al Cell Cycle 2014 Access via EMMA infrafrontier.eu/emma/strain-search/straindetails/?q=12167  
R26Arl13b-Fucci2aR Ford et al Dev Cell 2018 Access via EMMA infrafrontier.eu/emma/strain-search/straindetails/?q=12168  
R26Arl13b-Fucci2a Ford et al Dev Cell 2018 Access via EMMA infrafrontier.eu/emma/strain-search/straindetails/?q=12186