Researchers at MRC HGU under the leadership of Dr Richard Mort, conducted research into Fucci biosensors resulting in the development of the Fucci2a bicistronic and Arl13b-Fucci2a tricistronic sensors.
These biosensors have been a valuable research reagent and can be accessed as described below
Where to access Fucci biosensor reagents
Cell Lines | |||
| Material | Paper to be cited if used | Reference | Actions |
| Flp-NIH3T3 Fucci2a | Mort et al Cell Cycle 2014 | Via UoE MTA - contact Pleasantine Mill | PM to work with Richard Mort to ensure correct deposit of these cells and confirm with team |
| E14 MESC R26Fucci2aR | Mort et al Cell Cycle 2014 | Via UoE MTA - contact Pleasantine Mill | PM to work with Richard Mort to ensure correct deposit of these cells |
| E14 MESC R26Fucci2a | Mort et al Cell Cycle 2014 | Via UoE MTA - contact Pleasantine Mill | PM to work with Richard Mort to ensure correct deposit of these cells |
| Flp-NIH3T3 Arl13b-Fucci2a | Ford et al Dev Cell 2018 | Via UoE MTA - contact Pleasantine Mill | PM to work with Richard Mort to ensure correct deposit of these cells |
| E14 R26Arl13b-Fucci2aR | Ford et al Dev Cell 2018 | Via UoE MTA - contact Pleasantine Mill | PM to work with Richard Mort to ensure correct deposit of these cells |
| E14 R26Arl13b-Fucci2a | Ford et al Dev Cell 2018 | Via UoE MTA - contact Pleasantine Mill | PM to work with Richard Mort to ensure correct deposit of these cells |
Plasmids | |||
| Material | Paper to be cited if used | Location | Reference |
| pCAG-Fucci2a | Mort et al Cell Cycle 2014 | Access via RIKEN | dnaconda.riken.jp/search/RDB_clone/RDB13080 |
| pCDNA5-Fucci2a | Mort et al Cell Cycle 2014 | Access via RIKEN | dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16056 |
| pRosa26-CAG-floxNeo-Fucci2a | Mort et al Cell Cycle 2014 | Access via RIKEN | dnaconda.riken.jp/search/RDB_clone/RDB13/RDB13081 |
| pCAG-Arl13bCerulean-Fucci2a | Ford et al Dev Cell 2018 | Access via RIKEN | dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16059 |
| pCDNA5-CAG-Arl13bCerulean-Fucci2a | Ford et al Dev Cell 2018 | Access via RIKEN | dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16057 |
| pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a | Ford et al Dev Cell 2018 | Access via RIKEN | dnaconda.riken.jp/search/RDB_clone/RDB16/RDB16058 |
Mouse Lines | ||||
| Material | Paper to be cited if used | Location | Reference 1 | Reference 2 |
| R26Fucci2aR | Mort et al Cell Cycle 2014 | Access via RIKEN | infrafrontier.eu/emma/strain-search/straindetails/?q=8395 | knowledge.brc.riken.jp/resource/animal/card?brc_no=RBRC06511 |
| R26Fucci2a | Mort et al Cell Cycle 2014 | Access via EMMA | infrafrontier.eu/emma/strain-search/straindetails/?q=12167 | |
| R26Arl13b-Fucci2aR | Ford et al Dev Cell 2018 | Access via EMMA | infrafrontier.eu/emma/strain-search/straindetails/?q=12168 | |
| R26Arl13b-Fucci2a | Ford et al Dev Cell 2018 | Access via EMMA | infrafrontier.eu/emma/strain-search/straindetails/?q=12186 |