Access to Fucci reagents

How to access Fucci biosensor reagents developed at the Institute of Genetics and Cancer, University of Edinburgh.

Researchers at IGC under the leadership of Dr Richard Mort, conducted research into Fucci biosensors resulting in the development of the Fucci2a bicistronic and Arl13b-Fucci2a tricistronic sensors.

These biosensors have been a valuable research reagent and can be accessed as described below

Where to access Fucci biosensor reagents

Cell Lines

MaterialPaper to be cited if usedReferenceActions
Flp-NIH3T3 Fucci2aMort et al Cell Cycle 2014Via UoE MTA - contact Pleasantine MillPM to work with Richard Mort to ensure correct deposit of these cells and confirm with team
E14 MESC R26Fucci2aRMort et al Cell Cycle 2014Via UoE MTA - contact Pleasantine MillPM to work with Richard Mort to ensure correct deposit of these cells
E14 MESC R26Fucci2aMort et al Cell Cycle 2014Via UoE MTA - contact Pleasantine MillPM to work with Richard Mort to ensure correct deposit of these cells
Flp-NIH3T3 Arl13b-Fucci2aFord et al Dev Cell 2018Via UoE MTA - contact Pleasantine MillPM to work with Richard Mort to ensure correct deposit of these cells
E14 R26Arl13b-Fucci2aRFord et al Dev Cell 2018Via UoE MTA - contact Pleasantine MillPM to work with Richard Mort to ensure correct deposit of these cells
E14 R26Arl13b-Fucci2aFord et al Dev Cell 2018Via UoE MTA - contact Pleasantine MillPM to work with Richard Mort to ensure correct deposit of these cells

Plasmids

MaterialPaper to be cited if usedLocationReference
pCAG-Fucci2aMort et al Cell Cycle 2014Access via RIKENdnaconda.riken.jp/search/RDB_clone/RDB13080
pCDNA5-Fucci2aMort et al Cell Cycle 2014Access via RIKENdnaconda.riken.jp/search/RDB_clone/RDB16/RDB16056
pRosa26-CAG-floxNeo-Fucci2aMort et al Cell Cycle 2014Access via RIKENdnaconda.riken.jp/search/RDB_clone/RDB13/RDB13081
pCAG-Arl13bCerulean-Fucci2aFord et al Dev Cell 2018Access via RIKENdnaconda.riken.jp/search/RDB_clone/RDB16/RDB16059
pCDNA5-CAG-Arl13bCerulean-Fucci2aFord et al Dev Cell 2018Access via RIKENdnaconda.riken.jp/search/RDB_clone/RDB16/RDB16057
pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2aFord et al Dev Cell 2018Access via RIKENdnaconda.riken.jp/search/RDB_clone/RDB16/RDB16058

Mouse Lines

MaterialPaper to be cited if usedLocationReference 1Reference 2
R26Fucci2aRMort et al Cell Cycle 2014Access via RIKENinfrafrontier.eu/emma/strain-search/straindetails/?q=8395knowledge.brc.riken.jp/resource/animal/card?brc_no=RBRC06511
R26Fucci2aMort et al Cell Cycle 2014Access via EMMAinfrafrontier.eu/emma/strain-search/straindetails/?q=12167 
R26Arl13b-Fucci2aRFord et al Dev Cell 2018Access via EMMAinfrafrontier.eu/emma/strain-search/straindetails/?q=12168 
R26Arl13b-Fucci2aFord et al Dev Cell 2018Access via EMMAinfrafrontier.eu/emma/strain-search/straindetails/?q=12186