Forward Phase Antibody Microarrays

Our forward phase antibody microarray technology makes use of well validated capture/detection antibody pairs sourced from companies like R&D Systems for the relative quantification of secreted factors associated with inflammation and angiogenesis, e.g. chemokines/cytokines/adipokines. Combining the speed and accuracy of the ArrayJet Mercury 100 slide printer with the scanning power of the infra-red Innopsys Innoscan 710 allows us to profile cytokines & chemokines rapidly with high sensitivity.

• ELISA-based antibody array
• Fully customisable from a portfolio of >85 validated antibody pairs covering a range of cytokines, adipokines and growth factors
• Human and mouse antibody panels available
• Measure 16, 32 or 64 protein analytes
• Sample size: 20 to 80 samples depending on number of analytes being measured
• Can be run on samples from cell culture experiments, in vivo samples and human serum/plasma
• Further information

Unlike many commercial platforms we do not use detection antibody cocktails, instead favouring an approach whereby only the matching capture and detection antibodies are ever mixed.  This reduces non-specific signals that can arise by antibody crosstalk, which is a common cause of false positive results in these assays.