Details of our epifluorescent systems: Macred and Limelight, Britemac, Granny, Scumpy Calvados. Macred, Britemac and Limelight Image Macred epifluorescence microscope Modality Epifluorescence Fluorophores: DAPI, FITC, Texas Red, TRITC, Cy5 + BF Manufacturer Zeiss AxioImager 2 Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x Software Micro-Manager Application Fixed, colour brightfield, Britemac has an x,y,z scanning stage and is capable of tiling. Granny Image Granny epifluorescent microscope Modality Epifluorescence Fluorophores DAPI, FITC, Texas Red, TRITC, Cy5 + BF Manufacturer Zeiss AxioImager.A1 Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x Software Nikon NIS Application Fixed, z-stack/deconvolution Calvados and Scrumpy Image Scrumpy inverted epifluorescence microscope Modality Epifluorescence (Inverted), Live imaging, Conditional imaging (Scrumpy) Fluorophores DAPI, GFP, RFP, CFP, YFP + BF Manufacturer Zeiss Observer.Z1 (Calvados). Nikon Ti (Scrumpy) Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x Software Micro-Manager (Calvados), NIS Elements (Scrumpy) Application Fixed, brightfield, tiling, z-stack, live imaging This article was published on 2024-09-23