Resources and Downloads

Download sample submission guidelines, preparation protocols for proteomics, metabolomics, and lipidomics, and access our CASPA single-cell analysis pipeline on GitHub.

To ensure the highest data quality and a smooth project workflow, please review our submission guidelines and use our digital portal for all project requests.

1. Project Management & Booking (PPMS)

We use the Stratocore PPMS (Pasteur Platform Management System) for all sample tracking, instrument booking, and cost recovery.

2. Sample Submission Documents

Correct SampleSubissionForm_v3 (18.51 KB / XLSX)

Before delivering samples to the facility, please complete the following:

Includes: PI Information, Billing Codes, Sample IDs, Species, and Safety/Toxicity Declaration.

3. Sample Preparation Recommendations

Success in Mass Spectrometry begins at the bench. Please follow these core principles:

General Best Practices

Prepare the sample according to these protocols

IGC-Proteomics-Sample-Prep (33.38 KB / DOCX)

Laser Capture Microdissection--Proteomics, Deep Visual Proteomics (DVP)

IGC-DVP protocol (3.55 MB / DOCX)

Metabolomics/Lipidomics sample preparation protocols

Polar-Metabolites-Extraction (11.85 KB / DOCX)
PIP-Extraction (11.99 KB / DOCX)
Lipid-Saponification (23.74 KB / DOCX)
  • Purity: Don't add unnecessary detergents, polymers, Carrier Proteins, these can impact your analysis.
  • Plasticware/Glassware: Use only high-quality, "Low Protein Binding" tubes (e.g., Eppendorf LoBind) to prevent sample loss, use glass if you are working with aggressive solvents
  • Cleanliness: always work in a "contaminant-free" environment (fresh gloves, lab coat), avoid polymer/keratin/lipid contamination form skin/detergent/moisturiser etc.
  • Solvents: Make sure you use glass or PP tubes that are resistant to the solvents you are using.

Pillar-Specific Tips

Pillar Key Recommendation
Proteomics Flash-freeze cell pellets or tissue in liquid nitrogen. Aim for a minimum of 50 ug of protein for bulk analysis.
Metabolomics Quench metabolic activity immediately. Use chilled LC-MS grade solvents (Methanol/Acetonitrile) and keep samples on dry ice.
Lipidomics Avoid plasticizers where possible. For signaling lipids (e.g., PIP3), samples must be acidified and processed immediately.
Multiomics Do not split your sample. We use a specialized monophasic or biphasic extraction to recover all three layers from one tube. Contact us for the specific co-extraction protocol.

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